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STEMCELL Technologies Inc methylcellulose medium methocult m3134
Methylcellulose Medium Methocult M3134, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methocult+m3134+medium/methocult/pm39954676-242-8-10
Average 90 stars, based on 1 article reviews
methylcellulose medium methocult m3134 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: Homing and engraftment defects in ex vivo expanded murine hematopoietic cells are associated with downregulation of beta1 integrin.
Article Snippet: Objective.. Hematopoietic progenitors generated by ex vivo expansion “home” less efficiently to the bone marrow (BM) after intravenous transplantation than fresh cells.. To explore the underlying cause of this transplantation defect, we examined the homing and engraftment properties in vivo of fresh and cultured marrow cells differing in 1 integrin expression.

Recombinant:

Article Title: Homing and engraftment defects in ex vivo expanded murine hematopoietic cells are associated with downregulation of beta1 integrin.
Article Snippet: Objective.. Hematopoietic progenitors generated by ex vivo expansion “home” less efficiently to the bone marrow (BM) after intravenous transplantation than fresh cells.. To explore the underlying cause of this transplantation defect, we examined the homing and engraftment properties in vivo of fresh and cultured marrow cells differing in 1 integrin expression.

FACS:

Article Title: T-cell–Secreted TNFα Induces Emergency Myelopoiesis and Myeloid-Derived Suppressor Cell Differentiation in Cancer
Article Snippet: © 2018 American Association for Cancercancerres.aacrjournals.org Downloaded from AL SAYED et al T cell-secreted TNF-alpha activates myelopoiesis in cancer 7 isolated by positive magnetic cell sorting (MACS) of CD45+ cells using biotinylated-anti-CD45, MACS anti-biotin beads and LS columns (Miltenyi Biotec). .. FACS-sorted HSPCs, Lin- BM cells, splenocytes or blood cells were plated into MethoCult M3134 medium (STEMCELL Technologies) supplemented with 15% FCS, 20% BIT (50 mg/ml BSA in IMDM, 1.44 U/ml rh-insulin [Actrapid; Novo Nordisk], and 250 ng/ml human holo transferrin [Prospec]), 100 μM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine, and 50 ng/ml rmSCF, 10 ng/ml rm-IL-3, 10 ng/ml rh-IL-6, and 50 ng/ml rm-Flt3-ligand (Prospec). .. Colonies were counted after 7 days on a DMIL inverted microscope (Leica) equipped with an Intensilight C-HGFI unit (Nikon).

Cell Culture:

Article Title: Identification of clonogenic common Flt3+M-CSFR+ plasmacytoid and conventional dendritic cell progenitors in mouse bone marrow.
Article Snippet: Lymphoid tissue plasmacytoid and conventional dendritic cells (DCs) are continuously regenerated from hematopoietic stem cells.. The cytokine dependence and biology of plasmacytoid and conventional DCs suggest that regeneration might proceed through common DC-restricted developmental intermediates.. By selecting for cytokine receptor expression relevant to DC development, we identify here highly cycling Lin–c-KitintFlt3+M-CSFR+ cells with a distinct gene-expression profile in mouse bone marrow that, on a clonal level in vitro and as a population both in vitro and in vivo, efficiently generated plasmacytoid and conventional DCs but no other lineages, which increased in number after in vivo injection of the cytokine Flt3 ligand.



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